elysium symbolProtocols Outline:

Basic cell biology:  Transformation

Sterile Technique in BSL-2 Setting

Cell Culture, Passaging, and Well Sizes

Obtaining Plasmids(AddGene, Mini-prep, Outsourced Maxi prep)

How to view a Plasmid: SnapGene Viewer–Analyse Plasmid

Plasmid Concentration Calculations (How to use Spreadsheet Tool)

Mammalian Cell Transfection (Plasmids and  siRNA)

Lentivirus Production and Transduction

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